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New microsatellite loci for Narcissus papyraceus (Amarillydaceae) and cross-amplification in other congeneric species.

Identifieur interne : 000129 ( Main/Exploration ); précédent : 000128; suivant : 000130

New microsatellite loci for Narcissus papyraceus (Amarillydaceae) and cross-amplification in other congeneric species.

Auteurs : Violeta I. Sim N [Espagne] ; F Xavier Pic ; Juan Arroyo

Source :

RBID : pubmed:21622397

Abstract

UNLABELLED

PREMISE OF THE STUDY

Microsatellite loci from a genomic library of the species Narcissus papyraceus were optimized and characterized for studies of population genetics. •

METHODS AND RESULTS

Eleven markers that were successfully amplified showed polymorphism when tested on 50 individuals from two populations in southern Spain and northern Morocco. Overall, the number of alleles per locus ranged between 4 and 15. Between 8 and 11 loci successfully amplified in other eight Narcissus species. •

CONCLUSIONS

These markers will enable genetic diversity studies of N. papyraceus across its distribution range and conduct paternity analyses among individuals differing in flower morphology.


DOI: 10.3732/ajb.1000023
PubMed: 21622397


Affiliations:


Links toward previous steps (curation, corpus...)


Le document en format XML

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<name sortKey="Sim N, Violeta I" sort="Sim N, Violeta I" uniqKey="Sim N V" first="Violeta I" last="Sim N">Violeta I. Sim N</name>
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<nlm:affiliation>Departamento de Biología Vegetal y Ecología, Universidad de Sevilla, Sevilla, Spain.</nlm:affiliation>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>Departamento de Biología Vegetal y Ecología, Universidad de Sevilla, Sevilla</wicri:regionArea>
<wicri:noRegion>Sevilla</wicri:noRegion>
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<name sortKey="Pic, F Xavier" sort="Pic, F Xavier" uniqKey="Pic F" first="F Xavier" last="Pic">F Xavier Pic</name>
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<name sortKey="Arroyo, Juan" sort="Arroyo, Juan" uniqKey="Arroyo J" first="Juan" last="Arroyo">Juan Arroyo</name>
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<title level="j">American journal of botany</title>
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<b>UNLABELLED</b>
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<div type="abstract" xml:lang="en">
<p>
<b>PREMISE OF THE STUDY</b>
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<p>Microsatellite loci from a genomic library of the species Narcissus papyraceus were optimized and characterized for studies of population genetics. •</p>
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<div type="abstract" xml:lang="en">
<p>
<b>METHODS AND RESULTS</b>
</p>
<p>Eleven markers that were successfully amplified showed polymorphism when tested on 50 individuals from two populations in southern Spain and northern Morocco. Overall, the number of alleles per locus ranged between 4 and 15. Between 8 and 11 loci successfully amplified in other eight Narcissus species. •</p>
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<div type="abstract" xml:lang="en">
<p>
<b>CONCLUSIONS</b>
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<p>These markers will enable genetic diversity studies of N. papyraceus across its distribution range and conduct paternity analyses among individuals differing in flower morphology.</p>
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